Purification and properties of cytoplasmic granules from cytotoxic rat LGL tumors.

摘要: To evaluate the role of NK cell granules in lytic activity cells, cytoplasmic rat tumors were purified by centrifugation homogenates a Percoll gradient. Analysis such gradients showed band light-scattering material near bottom tube; assay gradient fractions for against SRBC potent giving sharp peak this region. Complete lysis was achieved with less than 1 microgram/ml protein most active fractions. Examination electron microscope that pool containing consisted pure showing similar morphology to those found LGL tumors. The associated four different lysosomal enzymes. marker enzymes mitochondria, plasma membrane, and cytosol well separated from peak. SDS gel electrophoresis granule fraction free contamination proteins other parts contained major bands 62, 58, 30, 29, 28 kilodaltons. In addition protein, contain hexose uronic acid, but no nucleic acids or phospholipids detected chemical assays. Major amounts chymotryptic, tryptic, elastase activities not present, nor peroxidase lysozyme detectable substantial amounts. These data show tumor have biochemical properties distinguish them present granulocytes mast cells.