Characterization of Monoclonal Antibodies Specific for Erwinia carotovora subsp. atroseptica and Comparison of Serological Methods for Its Sensitive Detection on Potato Tubers.

摘要: Seven monoclonal antibodies (MAbs) to Erwinia carotovora subsp. atroseptica have been produced. One, called 4G4, reacted with high specificity for serogroup I of E. atroseptica, the most common on potato tubers in different serological assays. Eighty-six strains belonging serogroups were assayed. Some XXII also positively. No cross-reactions observed against other species plant pathogenic bacteria or 162 saprophytic from tubers. Only one strain chrysanthemi cross-reacted. A comparison several techniques detect was performed MAb 4G4 polyclonal antibodies. The organism extracted directly peels artificially inoculated by soaking selective enrichment under anaerobiosis a medium polypectate. able specifically 240 cells per ml indirect immunofluorescence and colony staining after ELISA-DAS (double-antibody sandwich enzyme-linked immunosorbent assay) enrichment. same amount detected using immunolectrotransfer antibodies, distinguished latter technique. an step efficiently naturally infected plants.