Nitrile hydratase from Mesorhizobium sp F28 and its potential for nitrile biotransformation
作者： Yun-Shu Feng , Pei-Chung Chen , Fu-Shyan Wen , Wan-Yi Hsiao , Chi-Mei Lee
DOI: 10.1016/J.PROCBIO.2008.08.009
关键词:
摘要: Abstract Mesorhizobium sp. F28 was isolated from a wastewater treatment system in polyacrylonitrile (PAN) fibre factory for its ability to convert acrylonitrile by nitrile hydratase (NHase)–amidase (AMase) system. This strain completely degraded at concentrations as high 18.4 mM, and exhibited 192 U mg −1 dcw NHase activity the conversion of acrylamide. The cobalt-containing purified moderately thermostable, keeping full up 45 °C; it also had optimum between pH 7.0 7.5. Acrylonitrile, propionitrile, isobutyronitrile, benzonitrile were good substrates F28. affinity ( K m = 0.53 mM) greater than that bacteria mentioned other researchers before.
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