Identification and characterization of the pseudorabies virus UL3.5 protein, which is involved in virus egress.

摘要: Alphaherpesvirus genomes exhibit a generally collinear gene arrangement, and most of their genes are conserved among the different members subfamily. Among exceptions is UL3.5 pseudorabies virus (PrV) for which positional homologs have been detected in varicella-zoster virus, equine herpesvirus 1, bovine 1 but not herpes simplex types 2. To identify characterize predicted 224 amino acid protein PrV, rabbit antiserum was prepared against fusion expressed Escherichia coli. In Western blot (immunoblot) analyses 30-kDa cytoplasm PrV infected cells absent from purified virions. For functional analysis, UL3.5-expressing cell lines were established mutants isolated after rescue defective, glycoprotein B-negative by insertion complementing B-encoding at two sites within locus. A mutant carrying codon 159 expressing truncated still capable efficient productive replication noncomplementing cells. contrast, 10 did express detectable exhibited dramatic growth deficiency on non-complementing with regard to plaque formation one-step replication. Electron microscopical studies showed an accumulation unenveloped capsids vicinity Golgi apparatus. This defect could be compensated propagation lines. Our results thus demonstrate that encodes nonstructural plays important role replication, presumably during egress. The functionally relevant domains appear located N-terminal part also comprises region exhibiting highest level homology between homologous proteins other alphaherpesviruses