Succinate transport by free-living forms of Rhizobium japonicum.

摘要: We have demonstrated that the transport of succinate into cells Rhizobium japonicum strains USDA 110 and 217 is severely inhibited by cyanide, azide, 2,4-dinitrophenol, but not arsenate. These results suggest an active mechanism dependent on energized membrane, does directly utilize ATP. The apparent Km for was 3.8 microM strain 1.8 217; maximal velocities were 1.5 3.3 nmol per min mg protein, respectively. expression uptake activity inducible rather than constitutive, with structurally related compounds being most effective inducers. showed some specificity similar organic acids; fumarate L-malate classical competitive inhibitors system. In general, best competing also carbon substrates induction activity. EDTA succinate, implying a role divalent cations in When various used to reconstitute EDTA-inhibited activity, Ca2+ effective, followed Mg2+, which restored at about half efficiency Ca2+. Growth media supplemented increased concentration supported more rapid growth as substrate, from such higher specific activities transport.