A set of domain-specific markers in the Arabidopsis embryo

摘要: We describe a novel set of domain-specific markers that can be used in genetic studies, and we two examples to show loss stem cells a monopteros background. Multicellular organisms defined by their ability establish distinct cell identities, it is therefore critical importance distinguish types. One step leads identity specification activation unique sets transcripts. This property often exploited order infer identity; the availability good marker lines is, however, poor Arabidopsis embryo. Here (embryo) research. Based on transcriptomic data, selected 12 genes for expression analysis, according observed domain during embryogenesis, divided them into four categories (1—ground tissue; 2—root cell; 3—shoot apical meristem; 4—post-embryonic). additionally use from “stem cell” category study, where absence developmental defects monopteros mutant background. Finally, judge whether established also play role normal development, generated loss-of-function resources. None analyzed T-DNA insertion, artificial microRNA, or misexpression showed any apparent phenotypic difference wild type, indicating these are not nonredundantly required but suggesting considered an output patterning process. valuable addition currently available will help move toward generic tissue markers.