The Differential Mobilization of Histones H3.1 and H3.3 by Herpes Simplex Virus 1 Relates Histone Dynamics to the Assembly of Viral Chromatin

摘要: During lytic infections, HSV-1 genomes are assembled into unstable nucleosomes. The histones required for chromatin assembly, however, in the cellular chromatin. We have shown that linker (H1) and core (H2B H4) mobilized during infection, proposed available assembly viral However, actual relevance of histone mobilization remained unknown. now show canonical H3.1 variant H3.3 also infection. Mobilization no protein expression, although immediate early or proteins enhanced it. used previously known differential association with DNA to test mobilization. binds first, whereas only after replication initiates. Consistently, were differentially mobilized. decreased replication, was largely unaffected by These results support a model which is immobilized throughout mobilizations consistent their data therefore relate nuclear dynamics composition provide first evidence relates assembly.