A process for microbial hydrocarbon synthesis: Overproduction of fatty acids in Escherichia coli and catalytic conversion to alkanes.
作者： Rebecca M. Lennen , Drew J. Braden , Ryan M. West , James A. Dumesic , Brian F. Pfleger
DOI: 10.1002/BIT.22660
关键词:
摘要: The development of renewable alternatives to diesel and jet fuels is highly desirable for the heavy transportation sector, would offer benefits over production use short-chain alcohols personal transportation. Here, we report a metabolically engineered strain Escherichia coli that overproduces medium-chain length fatty acids via three basic modifications: elimination beta-oxidation, overexpression four subunits acetyl-CoA carboxylase, expression plant acyl-acyl carrier protein (ACP) thioesterase from Umbellularia californica (BTE). level BTE was optimized by comparing acid strains harboring on plasmids with different copy numbers. Expression low number resulted in highest production. Up seven-fold increase total observed negative control (lacking beta-oxidation), composition dominated C(12) C(14) saturated unsaturated acids. Next, strategy producing undecane combination biotechnology heterogeneous catalysis demonstrated. Fatty were extracted culture an overproducing into alkane phase fed Pd/C plug flow reactor, where decarboxylated alkanes. result enriched stream can be recycled continuous extractions. Complete conversion alkanes has been demonstrated yielding concentration 0.44 g L(-1) (culture volume) undecane.
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