Chapter 3 Membrane Nanotubes in Urothelial Cell Line T24

摘要: Abstract Membrane nanotubes (also referred as tunnelling nanotubes—TNTs, nanotubules, cytonemes), that directly connect separated neighboring cells, may offer a very specific and effective way of intercellular transport communication. Our experiments on T24 cell line show TNTs can be divided into two types with respect to their biochemical biophysical characteristics the nature formation. As type I were characterized which are shorter, more dynamic contain actin filaments. These structures remain stabile even if underlying cytoskeleton is disintegrated by cytochalasin D. The II much longer, appear stable cytokeratin In both microtubules found, but this present in only small fraction TNTs. On vesicular dilatations found an integral part (i.e., nanotubes, gondolas). Vesicular move along directions, while do not nanotubes. Both themselves transporting gondolas proposed involved communication transport. possible mechanisms stabilization membrane nanotubular protrusions also discussed.