Evaluation of Reference Genes for Quantitative Real-Time PCR in Oil Palm Elite Planting Materials Propagated by Tissue Culture

摘要: Background The somatic embryogenesis tissue culture process has been utilized to propagate high yielding oil palm. Due the low callogenesis and rates, molecular studies were initiated identify genes regulating process, their expression levels are usually quantified using reverse transcription quantitative real-time PCR (RT-qPCR). With recent release of palm genome sequences, it is crucial establish a proper strategy for gene analysis RT-qPCR. Selection most suitable reference should be performed accurate quantification levels. Results In this study, eight candidate selected from cDNA microarray study literature review evaluated comprehensively across 26 samples These collected two lines media treatments, which consisted leaf explants cultures, callus embryoids consecutive developmental stages. Three statistical algorithms (geNorm, NormFinder BestKeeper) confirmed that stability novel (pOP-EA01332, PD00380 PD00569) outperformed classical housekeeping (GAPDH, NAD5, TUBULIN, UBIQUITIN ACTIN). PD00569 identified as stably expressed in total samples, MA2 MA8 lines. Their applicability validate profiles putative ethylene-responsive factor 3-like demonstrated importance geometric mean normalization. Conclusions Systematic selection RT-qPCR was established samples. reliable normalization data will valuable research associated with process. Also, method described here facilitate appropriate other tissues profiling relating yield, biotic abiotic stresses.