Adsorption kinetics of bovine serum albumin on fused silica: population heterogeneities revealed by single-molecule fluorescence microscopy.

摘要: The adsorption of bovine serum albumin (BSA) on fused silica at neutral pH was investigated the single-molecule level by total internal reflection fluorescence microscopy. Dye-labeled BSA molecules that adsorbed quartz surface lit up as discrete, fluorescent dots which eventually disappeared upon desorption. Movies these events offered unprecedented details for kinetics modeling. results suggested 99.3% not sticky, and even if adsorbed, it would desorb in minutes. In contrast, remaining 0.7% only but anchor due course. Such population heterogeneity, otherwise masked ensemble measurements, sheds new light our understanding protein adsorption. methodology is also generally applicable to studies macromolecules interfaces.