Monoclonal antibodies to human alpha-interferon and their use for affinity chromatography.

摘要: A new screening procedure has been developed and used for the identification of three hybridomas secreting monoclonal antibodies to human leukocyte interferon (IFN-alpha). The is based on immune precipitation with a second antibody, acid dissociation precipitate, bioassay recovered interferon. This selects that are suitable affinity chromatography, as demonstrated by construction immunoadsorbents from these purification homogeneity in IFN-alpha.