Library construction, physical mapping, and sequencing

摘要: BAC Library Construction Kazutoyo Osoegawa and Pieter J. de Jong of Small Genome for Functional Genomic Applications David Smajs, Steven Norris, George M. Weinstock Preparation Libraries From Bacterial Genomes by In Vitro Packaging Sangita Pal, Solida Mak, Exploring Transformation-Associated Recombination Cloning Selective Isolation Regions Natalay Kouprina, Vladimir N. Noskov, Maxim Koriabine, Sun-Hee Leem, Larionov Purification DNA Tim S. Poulsen Hybridization-Based Selection Clones Chang-Su Lim Ung-Jin Kim Interspersed Repeat Sequence Polymerase Chain Reaction Heike Zimdahl, Claudia Gosele, Thomas Kreitler, Margit Knoblauch Mapping Using Fluorescence Situ Hybridization Xiao-Ning Chen Julie R. Korenberg High-Throughput Fingerprinting Jacqueline Schein, Tamara Kucaba, Mandeep Sekhon, Duane Smailus, Robert Waterston, Marco Marra End Sequencing Hans E. Johnsen Radiation Hybrid With Ends Michael Olivier, Shannon Brady, Cox Shotgun Bruce A. Roe Rolling Circle Amplification Templates Paul F. Predki, Chris Elkin, Hitesh Kapur, Jamie Jett, Susan Lucas, Tijana Glavina, Trevor Hawkins Transposon-Mediated Rachel Reeg Anup Madan Pyrosequencing: A Tool Analysis Elahe Elahi Mostafa Ronaghi Use Fimers to Eliminate Primer-Dimer Artifacts Increase Yield in BAC-Sequencing Reactions Andrei Malykh, Nikolai Polushin, Alexei Slesarev, Sergei Kozyavkin Retrofitting BACs PACs LoxP Transposonsto Generate Nested Deletions Pradeep K. Chatterjee Preparing Nested-Deletion Template Field Inversion Gel Electrophoresis Analyses Position-Specific Transposon Primers Joseph C. Baker, Jr. Finishing Strategies Christine Bird Darren Grafham the TIGR Assembler Projects Mihai Pop Dan Kosack 'Working Draft' Directed ThermoFidelase Olga Kozyavkin, Slesarev Optimized Multiplex Reaction: An Effective Method Rapid Gap Closure Diana Radune Herve Tettelin Assembly Data Jeremy Schmutz, Jane Grimwood, Richard Myers Quality Assessment Finished Sequences Index