I. Synthesis of anthraquinone derivatives for electron transfer studies in DNA. II. Characterization of the interaction between heme and proteins

摘要: Anthraquinone (AQ) derivatives with relatively high reduction potentials have been synthesized to afford good candidates for electron transfer studies in DNA. Electron withdrawing groups on the anthraquinone ring gave less negative potentials. The imide (AQI) had than AQ derivatives. AQI system was subject base-induced hydrolysis. Water-soluble sulfonated tetraarylporphyrins studied a wide variety of contexts. Herein, we report first synthesis pentasulfonated porphyrin bearing an internal cyclic sulfone ring. Treatment 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrin (TPPS4) fuming H2SO4 structure consistent initial sulfonation followed by dehydration give bridge between ortho-position one phenyl and β-pyrrole position porphine (TPPS4Sc). established ESI-MS 1 HNMR. Soret absorption is red shifted about 32 nm compared that TPPS4. Streptococcus pyogenes obtains iron taking up heme from environment during infection. One uptake pathways Sia or Hts pathway. protein this pathway Shr, which has two heme-binding NEAT domains, NEAT1 nearer N-terminus, NEAT2 C-terminus. We biophysical characteristics these domains. To assess stability domain towards release, denaturation Fe(II) Fe(III) forms were performed. For each domain, both behave similarly thermal guanidinium denaturation. Overall, more stable NEAT1. Spectral signatures, sequence alignment homology modeling domains suggest axial ligands methionine. autoreduces as pH increases autooxidizes decreases. Heme host only acquisition S. pyogenes; inhibition might be approach infection control. Compounds inhibit selected via virtual screening. INDEX WORDS: imide, DNA, transfer, Cyclic sulfone, porphyrin, Red shift, pyogenes, NEAT, Thermal denaturation, Guanidinium Ligand, Virtual I. SYNTHESIS OF ANTHRAQUINONE DERIVATIVES FOR ELECTRON TRANSFER STUDIES IN II. CHARACTERIZATION THE INTERACTION BETWEEN HEME AND PROTEINS.