Differential proteomic analysis of late-stage and recurrent breast cancer from formalin-fixed paraffin-embedded tissues.

摘要: The heterogeneity of breast cancer requires the discovery more incisive molecular tools that better define disease progression and prognosis. Proteomic analysis homogeneous tumor cell populations derived by laser microdissection from formalin-fixed, paraffin-embedded (FFPE) tissues has proven to be a robust strategy for conducting retrospective biomarker investigations. We describe an MS-based microdissected cancerous epithelial cells twenty-five patients at defined clinical stages with goal identifying protein abundance characteristics indicative recurrence. Comparative stage 0 III revealed 113 proteins significantly differentiated these groups included known factors associated pathogenesis, such as CDH1 CTNNB1, well those previously implicated in cancer, TSP-1. Similar analyses presenting II d...