Surface engineered antifouling optomagnetic SPIONs for bimodal targeted imaging of pancreatic cancer cells

摘要: Targeted imaging contrast agents for early pancreatic ductal adenocarcinoma diagnosis was developed using superparamagnetic iron oxide nanoparticles (SPIONs). For phase transfer of SPIONs, the hydrophobic SPIONs are first treated with tetrafluoroborate and then capped by bovine serum albumin (BSA) via ligand exchange. It experimentally found that nitrosyl pretreatment proper structures molecules essential to effective surface functionalization SPIONs. Nonspecific binding be significantly reduced BSA functionalized (BSA·SPIONs). The BSA·SPIONs were monodispersed an average size approximately 18.0 nm stable in a wide pH range various ionic strengths even after 7 days storage. longitudinal transverse proton relaxation rate (r1, r2) values determined 11.6 154.2 s(-1) per mM Fe(3+) respectively. r2/r1 ratio 13.3 ensured its application as T2-weighted magnetic resonance agents. When conjugated near-infrared fluorescent dye monoclonal antibody, (dye)BSA·SPION-monoclonal antibody bioconjugates showed excellent targeting capability minimal nonspecific bimodal cancer cells. experimental approach is facile, environmentally benign, straightforward, which presents great promise diagnosis.