Nattectin a fish C-type lectin drives Th1 responses in vivo: Licenses macrophages to differentiate into cells exhibiting typical DC function

摘要: Considerable efforts are currently focused on the biology of DC in view their possible clinical use as adjuvant for generation antigen-specific immunity and lifelong immunologic memory or treatment tumors. We assessed role Nattectin a C-type lectin identified Thalassophryne nattereri fish venom maturation. induced significant neutrophilic recruitment into peritoneal cavity mice, followed by macrophages, with lipidic mediators IL-12 p70 synthesis. Macrophages derived from 7day-Nattectin mice were CD11c+CD11b(low)Ly6(high)F4/80R(high) express high levels MHC class II CD80 molecules. Culture exudates macrophages 7day Nattectin-mice immature BMDCs markedly increased surface expression CD40, CD80, CD86, dose-dependent manner, production MMP-2 MMP-9 distributed nucleus cytoplasm cells, that was associated strong activity culture supernatant. treated DCs secreted IL-10. The Nattectin-treated BMDC macrophage-derived highly efficient at Ag capture. specific immune response elicited characterized antibodies IgG1 mainly IgG2a IL-10 IFN-γ synthesis splenic cells. These results enable us to address induces Ly6C(high) monocytes peritoneum, which exhibit pro-inflammatory profile, where they differentiate proliferating F4/80R(high) macrophages. Macrophage-derived mature presence cytokine milieu generated against Nattectin, exhibiting T cell co-stimulatory molecule Th1 polarized response.