Insulin binding to target cells

摘要: Following Yalow and Berson's basic research on the binding of polvpeptide hormones to plasma proteins, an integrated picture hormone-receptor interaction biological activity has been proposed for insulin in experimental models man. The extracellular molecule with cell membrane structure modifies intracellular metabolism, it suggested that this occurs through activation a second messenger or trans. duction fragment into cell. use monoiodoinsulin made possible perform series experiments cells isolated from blood (monocytes) tissues (adipocytes) membranes prepared by ultracentrifugation. existence specific receptor sites all cases, both animals man, confirmed mathe matical analysis curves; their non-linear course as plotted Scatchard's method, may depend negative cooperation different classes receptor. From evaluation recent studies human obesity, particularly adipocytes circulating monocytes, new approach problem ‘insulin resistance’ obesity proposed, shown reduction number receptors target contribute peripheral insensitivity. This phenomenon, which seems be characteristic static phase is reversible during fasting weight compensatory mechanism hyper-insulinaemia. These results are example significance applicability method detecting cells, suggest wide application endocrinological fields.