Production of cloned calves by combination treatment of both donor cells and early cloned embryos with 5-aza-2/-deoxycytidine and trichostatin A

摘要: We previously reported that treatment of both donor cells and early cloned embryos with a combination 0.01 μM 5-aza-2(/)-Deoxycytidine (5-aza-dC) 0.05 trichostatin A (TSA) significantly improved development bovine in vitro. In the present study, we investigated effect this on vivo potency postnatal survivability calves. Blastocysts (77 82 blastocysts derived from untreated (control) treated groups, respectively) were individually transferred to recipient cows. Relative control group, 5-aza-dC TSA dramatically increased cleavage rate (49.2 vs 63.6%, P < 0.05) at 24 h culture, blastocyst Days 6 7 culture (18.8 33.9% 27.1 38.5% respectively, 0.05). Although pregnancy did not differ 40 d after transfer, it was lower than group 90 transfer (7.8 29.3%, there three calves born 77 recipients (only two survived beyond 60 d), whereas 17 recipients, 11 d. conclusion, enhanced somatic cell vivo; cloning efficiency (number surviving birth/number cows) 2.6 13.4%.