The effect of initial pore volume and lignin content on the enzymatic hydrolysis of softwoods

作者: Caitriona A. Mooney , Shawn D. Mansfield , Maria G. Touhy , John N. Saddler

DOI: 10.1016/S0960-8524(97)00181-8

关键词:

摘要: Four Douglas-fir pulps, a refiner mechanical pulp (RMP), sulphonated RMP, delignified RMP and kraft were used to determine whether the lignin content initial pore volume affected cellulase adsorption substrate hydrolysis. When compared on basis of content, it was apparent from treatment that proportion did not affect enzyme when fibres sufficiently swollen. However, observed does always translate fast complete Pore data revealed although modification resulted in dramatically increased fibre saturation point median width accordingly. In contrast, had higher hydrolyzed more completely, suggesting steric hindrance residual may be rate limiting characteristic this situation. Hydrolysis indicated larger average particle size might have been an inhibiting factor, since slowly than despite having lower content.

参考文章(19)
Dora Lee, Alex H. C. Yu, Ken K. Y. Wong, John N. Saddler, Evaluation of the enzymatic susceptibility of cellulosic substrates using specific hydrolysis rates and enzyme adsorption Applied Biochemistry and Biotechnology. ,vol. 45, pp. 407- 415 ,(1994) , 10.1007/BF02941815
D. S. Burns, H. Ooshima, A. O. Converse, Surface area of pretreated lignocellulosics as a function of the extent of enzymatic hydrolysis Applied Biochemistry and Biotechnology. ,vol. 20, pp. 79- 94 ,(1989) , 10.1007/BF02936474
Anette Heijnesson, Rune Simonson, Ulla Westermark, Removal of lignin-rich surface material from unbleached kraft fibres Holzforschung. ,vol. 49, pp. 313- 318 ,(1995) , 10.1515/HFSG.1995.49.4.313
L. P. Ramos, C. Breuil, J. N. Saddler, Comparison of steam pretreatment of eucalyptus, aspen, and spruce wood chips and their enzymatic hydrolysis Applied Biochemistry and Biotechnology. ,vol. 34, pp. 37- 48 ,(1992) , 10.1007/BF02920532
H. E. Grethlein, D. C. Allen, A. O. Converse, A comparative study of the enzymatic hydrolysis of acid-pretreated white pine and mixed hardwood Biotechnology and Bioengineering. ,vol. 26, pp. 1498- 1505 ,(1984) , 10.1002/BIT.260261215
J. E. Stone, A. M. Scallan, Effect of component removal upon the porous structure of the cell wall of wood Journal of Polymer Science Part C: Polymer Symposia. ,vol. 11, pp. 13- 25 ,(1965) , 10.1002/POLC.5070110104
L. T. Fan, Yong-Hyun Lee, David H. Beardmore, Mechanism of the enzymatic hydrolysis of cellulose: Effects of major structural features of cellulose on enzymatic hydrolysis Biotechnology and Bioengineering. ,vol. 22, pp. 177- 199 ,(1980) , 10.1002/BIT.260220113
L.P. Ramos, M.M. Nazhad, J.N. Saddler, Effect of enzymatic hydrolysis on the morphology and fine structure of pretreated cellulosic residues Enzyme and Microbial Technology. ,vol. 15, pp. 821- 831 ,(1993) , 10.1016/0141-0229(93)90093-H