Programmed synthesis of three-dimensional tissues

摘要: Reconstituting tissues from their cellular building blocks facilitates the modeling of morphogenesis, homeostasis and disease in vitro. Here we describe DNA-programmed assembly cells (DPAC), a method to reconstitute multicellular organization organoid-like having programmed size, shape, composition spatial heterogeneity. DPAC uses dissociated that are chemically functionalized with degradable oligonucleotide 'Velcro', allowing rapid, specific reversible cell adhesion other surfaces coated complementary DNA sequences. DNA-patterned substrates function as removable adhesive templates, layer-by-layer builds arrays into third dimension above template. DNase releases completed microtissues template concomitant full embedding variety extracellular matrix (ECM) gels. positions subpopulations single-cell resolution generates cultures several centimeters long. We used explore impact ECM composition, heterotypic cell-cell interactions patterns signaling heterogeneity on collective behaviors.