The unique role of interferon-gamma in the regulation of MHC expression on arterial endothelium.

摘要: We examined the expression of MHC class I and II in arterial endothelium interferon-γ (IFN-γ, GKO) IFN-γ-R (IFN-γ-R, GRKO) gene knockout mice comparison with intact IFN-γ genes, BALB/c 129Sv/J wild-type, respectively. The GKO GRKO were produced by targeting. was assessed mAb binding to frozen tissue (kidney, spleen, heart, liver) sections immunoperoxidase staining basal state after various stimuli: allogeneic cells, oxazolone skin sensitization, LPS, rIFN-γ. As controls, we also two other inducible genes present endothelium, Ly-6 ICAM-1. found that small arteries arterioles wild-type but absent from mice. Class induced three vivo allogeneic, oxazolone, whereas only stimulus. Administration rIFN-γ ICAM-1 similar mice, indicating that, these proteins is independent, unlike I. In summary, not constitutive as previously believed, dependent on production. has an essential role induction endothelium. fact may be useful therapeutically for reduction immune recognition transplantation.