From iron to antibiotics: Identification of conserved bacterial-fungal interactions across diverse partners

摘要: Microbial interactions are major determinants in shaping microbiome structure and function. Characterization of these is not only essential to improve our understanding ecology microbiology general, but also identify novel molecules or processes with potential biotechnological applications. Interkingdom such as bacterial-fungal have been shown be particularly important microbial ecosystems. However, the diversity conservation remain poorly characterized most studies so far focused on specific bacterium-fungus pairs. In this work, we explore by characterizing molecular interaction mechanisms 16 different pairs, involving 8 fungi 2 bacteria (Escherichia coli Pseudomonas psychrophila). Using random barcode transposon site sequencing (RB-TnSeq), identified bacterial genes pathways for survival fungal contexts. We found a wide interactions, both antagonistic beneficial. Interestingly, observe set that appear conserved across partners studied. Our data suggest hydroxamate siderophores alleviate need endogenous siderophore uptake. addition, using mutant master regulator secondary metabolite production, metabolites key shapers fitness profiles. The diverse indicate that, addition many species-specific effects, may microbiomes.