Sex Determination of Sika Deer (Cervus nippon yesoensis) Using Nested PCR from Feces Collected in the Field
作者： Shoki YAMAZAKI , Yuta MOTOI , Kazuya NAGAI , Tsuyoshi ISHINAZAKA , Makoto ASANO
DOI: 10.1292/JVMS.11-0235
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摘要: We describe a method for determining the sex of sika deer (Cervus nippon yesoensis) from feces collected in the field. Using a nested polymerase chain reaction (nested PCR), partial sequences of the sex determination region of the Y chromosome (SRY) gene and X zinc finger protein (ZFX) gene were amplified. In 19 individuals with sex information, the correct sex was successfully detected and sequences of target amplicons were completely matched between muscle and feces from the rectum. Among 75 fecal samples collected …
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