Resolution of poxvirus telomeres: processing of vaccinia virus concatemer junctions by conservative strand exchange.

摘要: The replication of vaccinia virus proceeds through concatemeric intermediates which are resolved into unit-length DNA. In virus-infected cells, plasmids containing the DNA junction fragment that connects concatemers linear minichromosomes vector flanked by hairpin loops. Resolution requires two copies a specific nucleotide sequence conserved among poxviruses and found proximal to loop. This study demonstrates orientation each with respect other as well axis symmetry is critical for resolution, processing heterologous pairs resolution sites influenced mismatched nucleotides between sites, in minichromosome heteroduplex composed from strand concatemer junction. A model incorporating site-specific recombination orientated branch migration proposed account