Gibberellic acid stabilises microtubules in maize suspension cells to cold and stimulates acetylation of α-tubulin 1
作者： Rong Feng Huang , Clive W Lloyd
DOI: 10.1016/S0014-5793(98)01718-9
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摘要: Gibberellic acid is known to stabilise microtubules in plant organs against depolymerisation. We have now devised a simplified cell system for studying this. Pretreatment of maize suspension with gibberellic just 3 h stabilised protoplast depolymerisation on ice. In other eukaryotes, acetylation α-tubulin correlate microtubule stabilisation but this not established plants. By isolating the polymeric tubulin fraction from cytoskeletons and immunoblotting antibody 6-11B-1, we demonstrated that stimulates α-tubulin. This first link between higher
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