Integrated analysis of hydrothermal flow through pretreatment

摘要: Background: The impact of hydrothermal flowthrough (FT) pretreatment severity on and solubilization performance metrics was evaluated for three milled feedstocks (corn stover, bagasse, poplar) two conversion systems (simultaneous saccharification fermentation using yeast fungal cellulase, by Clostridium thermocellum). Results: Compared to batch pretreatment, FT consistently resulted in higher XMG recovery, removal non-carbohydrate carbon glucan simultaneous (SSF). recovery above 90% below 4.1 but decreased at severities, particularly bagasse. Removal during increased from 65% low 80% high corn 40% 70% bagasse poplar. Solids obtained were amenable all the examined. optimal time temperature poplar found be 16 min 210°C. At these conditions, SSF about 85%, 94% removed, 62% non carbohydrate mass solubilized. Solubilization FT-pretreated compared C. thermocellum (10% inoculum), yeast-fungal cellulase (5% inoculum, loading 5 10 FPU/g supplemented with β-glucosidase 15 30 U/g glucan). Under conditions tested, which featured solids concentration, achieved faster rates more complete than did SSF. SSF, 30% after 4 days, over twice as fast ball-milled Conclusions: trends similar between whereas significantly different, suggesting that factors addition amenability enzymatic attack. Corn stover exhibited hydrolysis yields or poplar, could due carbon. is easily degraded Conversion substrates concentration