Characterization of Endophytic Fungi from Acer ginnala Maxim. in an Artificial Plantation: Media Effect and Tissue-Dependent Variation

摘要: The community of endophytic fungi associated with Acer ginnala, a common tree in northeastern China, was investigated. Four media, PDA, Czapek’s, WA and Sabouraud’s, were used to inoculate explants from seeds, annual twigs perennial (xylem bark). Media strongly affected the isolated species number, but not colonization frequency (CF) or isolation (IF). To investigate media effect further, Principal Component Analysis (PCA) done. As result, two components accounted for 86.502% total variance extracted. These named as PDA-determined factor (accounted 45.139% variance) Czapek’s-determined 41.363% variance), respectively. This result suggested that only PDA could be instead all four this study without affecting results significantly. In total, ten taxa study. Alternaria sp., Phomopsis Neurospora sp. Phoma dominant endophytes while Pleosporales Incertae Sedis Cladosporium Trichoderma Epicoccum rare taxa. Different tissues/organs had different endophyte assemblages. All tissue/organ pairs low Bray-Curtis indices (<0.3) except bark (0.63). Compared twigs, lower taxon isolate dominance diversity indices. Seeds distinct assemblage, similarity similar twigs. tissue type determines assemblage age diversity.