DAPK1 mediates the G1 phase arrest in human nasopharyngeal carcinoma cells induced by grifolin, a potential antitumor natural product
作者： Xiang-jian Luo , Wei Li , Li-fang Yang , Xin-fang Yu , Lan-bo Xiao
DOI: 10.1016/J.EJPHAR.2011.08.026
关键词:
摘要: Grifolin, a secondary metabolite isolated from the fresh fruiting bodies of mushroom Albatrellus confluens, has been shown to induce Cl phase cell-cycle arrest in tumor cells previous studies our group. However, mechanisms action are not completely understood. Our group further demonstrated that grifolin upregulates death-associated protein kinase 1 (DAPK1) nasopharyngeal carcinoma (NPCs). Here, we found induced dephosphorylation DAPK1 (Ser308) activate and subsequent phosphorylation its potential downstream effector p21 (Thr145) cell CNE1. Inhibition by introducing siRNA targeting reversed grifolin- p21. Furthermore, confirmed increased half-life promoted stability. Flow cytometry analysis was involved grifolin-induced CNE1 cells. The similar effects mechanism beneath were identified another HONE1. In addition, observed protein-protein interaction ERK1/2 prevent nucleolus translocation. findings indicate plays crucial role induction at grifolin. Grifolin might represent promising candidate prevention intervention cancer signaling cycle arrest. (C) 2011 Elsevier B.V. All rights reserved.
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