Leishmania major: Production of recombinant gp63, its antigenicity and immunogenicity in mice

摘要: The Mr 63,000 membrane polypeptide (gp63) is one of the Leishmania receptors for host macrophages and has been shown to protect mice from infection. gene encoding gp63, major surface glycoprotein L. promastigotes, expressed as a fusion protein with enzyme glutathione S-transferase encoded by parasitic helminth Schistosoma japonicum. This was recognized polyclonal antibodies native gp63 polypeptide. insoluble purified SDS-PAGE electroelution used raise in rabbits. These rabbit anti-gp63 denatured parasite on immunoblots immunofluorescence fixed but did not recognize molecule live organisms. However, raised against promastigote glycoproteins, affinity solid-phase protein, both suggesting presence determinants recombinant protein. either healer or nonhealer phenotype challenge infection promatigotes. implications these results engineering DNA-produced molecular vaccines are discussed.