Self-organization and branching morphogenesis of primary salivary epithelial cells.

摘要: Embryonic tissues may provide clues about mechanisms required for tissue reassembly and regeneration, but few studies have utilized primary embryonic to study assembly. To test the capacity of fragments regenerate, we cultured day 13 (E13) mouse submandibular gland (SMG) epithelium found that as small a quarter-bud retain ability branch. Further, completely dissociated SMG epithelial cells self-organize into structures undergo significant branching. Investigation involved in self-assembly demonstrated inhibition beta(1) integrin prevents cell aggregation, while E-cadherin hinders aggregate compaction. Immunostaining showed cellular architecture expression patterns E-cadherin, beta-catenin, actin reassembled aggregates mirror those seen intact glands. Adding mesenchymal cultures facilitates branching morphological differentiation. Quantitative real-time RT-PCR indicated express differentiation markers aquaporin-5 (AQP5), prolactin-inducible protein (PIP), C (SMGC). Together, these data show morphogenesis form with structural features characteristic gland. These findings insights will facilitate future regeneration strategies salivary other organs.