Solution properties and chain flexibility of pullulan in aqueous solution

摘要: Molecular characteristics for pullulan, a polysaccharide produced by fungus Aureobasidium pullulans, were measured light scattering, viscometry, and gel-permeation chromatography. From the experimental data Mark-Houwink-Sakurada viscosity equation in water at 25°C was determined samples having molecular weight M ranging from 48 × 103 to 2.18 106 g mol−1 as [η] = (1.91 ± 0.02) 10−2Mw0.67±0.01 (in cm3 g−1); decreased, slope of although values below 30 evaluated chromatography somewhat unreliable. The unperturbed dimensions 〈R2〉01/2 pullulan estimated determining expansion factor αs, theoretical combination theories interpenetration function Ψ those αs. 〈R2〉0/M value this procedure 6.7 10−17 cm2 mol g−1. We concluded that chain is linked α-1,6-glucosidic linkage behaves like flexible aqueous solution.