Use of particle beam liquid chromatography—electron impact mass spectrometry for structure elucidation of oxodipine and three of its metabolites

摘要: In order to test the analytical capabilities of particle beam liquid chromatograph-mass spectrometer interface in structural identification drug metabolism, a chromatographic-mass spectrometric (LC MS) method using this new technique was developed for oxodipine and some its expected metabolites. After two extraction steps at pH 9 1.5, separation compounds, which have wide polarity range, carried out by an isocratic high-performance chromatographic with 25-cm cyano-bonded column. The compounds were eluted hexane-methanol-methylene chloride (76:12:12). Mass spectra recorded after electron impact ionization (75 eV) source temperature 150 degrees C. Under these conditions, comparison those obtained gas chromatography or direct introduction probe showed identical fragmentation patterns when sufficient amount product injected LC-MS analysis.