Polymorphism and Drug-Selected Mutations in the Protease Gene of Human Immunodeficiency Virus Type 2 from Patients Living in Southern France

摘要: The susceptibility of human immunodeficiency virus type 2 (HIV-2) to protease inhibitors (PI) is largely unknown. We studied HIV-2 genes from 21 HIV-2-infected patients who were exposed or not PI. aim this study was (i) characterize the polymorphism in absence drug, (ii) know whether gene naturally harbors HIV-1 drug resistance codons, and (iii) identify mutations emerging under PI-selective pressure. Sixty-five RNA proviral DNA samples directly sequenced plasma peripheral blood mononuclear cells 8 had received PI 13 never any antiretroviral. In untreated patients, highest amino acid variability observed at positions 14, 40, 43, 46, 65 70, seven codons (10V, 32I, 36I, 46I, 47V, 71V, 73A) associated with highly prevalent. addition, six (positions 7, 62, 71, 90, 99), frequencies both differed significantly PI-treated suggesting that 7K→R, 46V→I, 62V→A/T, 71V→I, 90L→M 99L→F occurring At these positions, least one sample simultaneously harbored wild-type mutated while substitutions 99 confirmed a longitudinal analysis. Moreover, presence 46I 99F subtype B proteases may reflect natural conclusion, present revealed strains harbor specific patterns resistance.