The effects of modeled microgravity on growth kinetics, antibiotic susceptibility, cold growth, and the virulence potential of a Yersinia pestis ymoA-deficient mutant and its isogenic parental strain.
作者： Abidat Lawal , Michelle L. Kirtley , Christina J. van Lier , Tatiana E. Erova , Elena V. Kozlova
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摘要: Abstract Previously, we reported that there was no enhancement in the virulence potential (as measured by cell culture infections) of bacterial pathogen Yersinia pestis (YP) following modeled microgravity/clinorotation growth. We have now further characterized effects clinorotation (CR) on YP growth kinetics, antibiotic sensitivity, cold growth, and YP's a murine model infection. Surprisingly, none aforementioned phenotypes were altered. To better understand why CR did not enhance as it for other pathogens, ΔymoA isogenic mutant KIM/D27 background strain is unable to produce histone-like YmoA protein influences DNA topography used both models represses type three secretion system (T3SS) gene expression yersiniae. Similar our CR-grown parental data, induced reduced HeLa cytotoxicity with con...
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