Close association of RNA polymerase II and many transcription factors with Pol III genes

摘要: Transcription of the eukaryotic genomes is carried out by three distinct RNA polymerases I, II, and III, whereby each polymerase thought to independently transcribe a set genes. To investigate possible relationship II we mapped their in vivo binding sites throughout human genome using ChIP-Seq two different cell lines, GM12878 K562 cells. Pol III was found bind near many known genes as well several previously unidentified target RNA-Seq studies indicate that majority bound are expressed, although subset not suggestive stalling III. genes, including tRNA, U6, HVG, hY, 7SK Similarly, also reveal number transcription factors normally associated with transcription, c-Fos, c-Jun c-Myc, tightly associate most III-transcribed Inhibition activity α-amanitin reduced expression (e.g., HVG), suggesting plays an important role regulating transcription. These results that, contrary previous expectations, can often work one another globally coordinate gene expression.