Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity

摘要: Pseudomonas aeruginosa san ai, an alkaliphilic, metallotolerant bacterium, degraded individual selected petroleum compounds, i.e., n-alkanes (n-hexadecane, n-nonadecane) and polycyclic aromatic hydrocarbons (fluorene, phenanthrene, pyrene) with efficiency of 80%, 98%, 96%, 50% 41%, respectively, at initial concentrations 20 mg L−1 in seven days. P. ai showed a high biodegradative capacity on complex hydrocarbon mixtures, the aliphatic fractions from crude oil. The degradation oil days reached stage 3–4 biodegradation scale, which ranges 0 (no biodegradation) to 10 (maximum biodegradation). Identified metabolites concomitant genomic enzymatic data indicated terminal oxidation pathway for n-alkane degradation, salicylate phthalate pathways fluorene biodegradation. Polyextremophilic as biosurfactant producer multidegradative hydrocarbons, can be used improved strategy environmental bioremediation hydrocarbon-contaminated sites, including extreme habitats characterized by low or elevated temperatures, acidic alkaline pH heavy metals.