G-quadruplexes Significantly Stimulate Pif1 Helicase-catalyzed Duplex DNA Unwinding
作者： Xiao-Lei Duan , Na-Nv Liu , Yan-Tao Yang , Hai-Hong Li , Ming Li
关键词:
摘要: The evolutionarily conserved G-quadruplexes (G4s) are faithfully inherited and serve a variety of cellular functions such as telomere maintenance, gene regulation, DNA replication initiation, epigenetic regulation. Different from the Watson-Crick base-pairing found in duplex DNA, G4s formed via Hoogsteen base pairing very stable compact structures. Failure untangling them cell impedes DNA-based transactions leads to genome instability. Cells have evolved highly specific helicases resolve G4 We used recombinant nuclear form Saccharomyces cerevisiae Pif1 characterize Pif1-mediated unwinding with substrate mimicking an ongoing lagging strand synthesis stalled by G4s, which resembles origin G4-structured flap Okazaki fragment maturation. find that presence may greatly stimulate helicase unwind DNA. Further studies reveal this stimulation results G4-enhanced dimerization, is required for unwinding. This finding provides new insights into properties G4s. discuss observed activation phenomenon relation possible regulatory role rapid rescue helping replicator recognize activate well quickly removing during
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