Regulation of insulin gene transcription by the immediate-early growth response gene Egr-1.
作者： Kazuhiro Eto , Varinderpal Kaur , Melissa K. Thomas
DOI: 10.1210/EN.2005-1336
关键词:
摘要: Changes in extracellular glucose levels regulate the expression of immediate-early response gene and zinc finger transcription factor early growth response-1 (Egr-1) insulin-producing pancreatic β-cells, but key target genes Egr-1 endocrine pancreas have not been identified. We found that overexpression clonal (INS-1) β-cells increased transcriptional activation rat insulin I promoter. In contrast, reductions or function with introduction either small interfering RNA targeted to (siEgr-1) a dominant-negative form decreased promoter activation, siEgr-1 suppressed expression. did directly interact sequences, mutagenesis potential G box recognition sequence for impair responsiveness promoter, suggesting regulation by is probably mediated through additional factors. Ov...
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