Apigenin-7-O-β-D-glucuronide inhibits LPS-induced inflammation through the inactivation of AP-1 and MAPK signaling pathways in RAW 264.7 macrophages and protects mice against endotoxin shock

作者: Weicheng Hu , Xinfeng Wang , Lei Wu , Ting Shen , Lilian Ji

DOI: 10.1039/C5FO01212K

关键词:

摘要: Apigenin-7-O-β-D-glucuronide (AG), an active flavonoid derivative isolated from the agricultural residue of Juglans sigillata fruit husks, possesses multiple pharmacological activities, including anti-oxidant, anti-complement, and aldose reductase inhibitory activities. To date, no report has identified anti-inflammatory mechanisms AG. This study was therefore designed to characterize molecular AG on lipopolysaccharide (LPS)-induced inflammatory cytokines in RAW 264.7 cells endotoxin-induced shock mice. suppressed release nitric oxide (NO), prostaglandin E2 (PGE2), tumour necrosis factor-α (TNF-α) LPS-stimulated macrophages a dose-dependent manner without affecting cell viability. Additionally, LPS-induced mRNA expression inducible synthase (iNOS), cyclooxygenase-2 (COX-2), TNF-α. treatment decreased translocation c-Jun into nucleus, activator protein-1 (AP-1)-mediated luciferase activity through inhibition both p38 mitogen-activated protein kinase (MAPK) extracellular signal-regulated (ERK) phosphorylation. Consistent with vitro observations, protected mice endotoxin by inhibiting proinflammatory cytokine production. Taken together, these results suggest that may be used as source agents well dietary complement for health promotion.

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