Induction of M2 Polarization in Primary Culture Liver Macrophages from Rats with Acute Pancreatitis

摘要: Background and Aims Systemic inflammatory response syndrome (SIRS), a major process of severe acute pancreatitis (SAP), usually occurs after various activated proinflammatory cytokines, which are produced by macrophages such as liver macrophages. Macrophages can secrete not only mediators but also inhibitory cytokines IL-10, leading to two different functional states defined “polarization”. The main purpose this study was demonstrate the polarization during explore whether these Liver could be reversed in vitro. Methods Liver were isolated from rats with pancreatitis. These primary culture treated IL-4 or regulatory T cells vitro reverse their evaluated measuring M1/M2 marker expression using real time PCR immunofluorescence staining. Results Acute induced successfully intra-pancreatic ductal injection 5% sodium taurocholate. demonstrated M1 4 h 16 onset However, Treg treatment, indicated increased M2 markers reduced markers. Furthermore, effect on modulating macrophage slightly better than that vitro. Conclusion macrophages, pivotal cell type pathogenesis SAP, become polarized pancreatic inflammation. Treatment activation vitro. This method altering prospective therapy for SAP.