Ultrastructural and biochemical studies of two dynamically expressed cell surface determinants on Candida albicans.

摘要: Abstract Variability in the expression of two different cell surface carbohydrate determinants was examined with agglutinating immunoglobulin M monoclonal antibodies (H9 and C6) immunoelectron microscopy during growth three strains Candida albicans. A single strain parapsilosis did not express either antigen at any time growth. Antigens were detected on C. albicans by agglutination tests H9 or C6 over a 48-h period. The difference specificities demonstrated Ouchterlony double-diffusion solubilized antigens variabilities reactivity agglutinins among yeast strains. antigenic isolated specific immunoprecipitation protease digestion characterized methods including high-pressure liquid chromatography, gas-liquid mass spectroscopy both chemical electron ionization. These contain mannose glucose. In case H9, an additional gas chromatography spectroscopy. location individual cells determined indirect labeling determinants, first reacting followed goat anti-mouse antibody conjugated 20-nm colloidal gold particles. Transmission used to examine cells. that reactive associated flocculent layer. Expression this layer is dynamic process which phase dependent. expressed very young disappeared from most age. use may allow characterization be helpful establishing receptors mediate adherence.