α6β1-Integrin, a Major Cell Surface Carrier of β1-6-branched Oligosaccharides, Mediates Migration of EJ-ras-transformed Fibroblasts on Laminin-1 Independently of Its Glycosylation State

摘要: Abstract EJ- ras oncogene-induced malignant transformation is characterized by a series of changes in cell surface carbohydrates and cell-cell cell-matrix interactions. Here, we show that -transformed NIH-3T3 fibroblasts acquired migratory phenotype on laminin-1 surfaces. Such was accompanied overexpression of: ( ) functional α6β1, but not other laminin binding β1-integrins; b glycoconjugates the bearing large oligosaccharides recognized leukoagglutinin from Phaseolus vulgaris (L-PHA). The internal pool pre-β1-integrins differently regulated cells compared with nontransfected fibroblasts. Conversion pre-β1- into mature β1-integrins faster cells, process associated α6-chain. Overexpression L-PHA-reactive dependent activity N -acetylglucosaminyltransferase V, which increased transformed [J. W. Dennis et al. , Science (Washington DC), 236: 582–585, 1987]. We were major carriers surface. This glycosylation pattern, however, necessary for either expression or their response to laminin-1. Moreover, aggregated spontaneously. These effects observed c- jun -transfected fibroblasts, unable migrate laminin, did overexpress oligosaccharides, self-aggregate.