Viable but Nonculturable Cells in Plant-Associated Bacterial Populations

摘要: Plants in terrestrial ecosystems are colonized by complex microbial communities composed of bacteria, yeasts, and filamentous fungi. These have received much attention because their effects on plant productivity. While these contain some deleterious organisms, such as phytopathogenic bacteria fungi, they also beneficial nitrogen-fixing capable suppressing disease, promoting growth. Quantification bacterial populations plantassociated epidemiological, pathological, ecological studies has to date relied almost exclusively upon either plate counts using selective media (4, 48, 87) or quantitative immunofluorescence microscopy (13, 28). Only recently techniques for the quantification viable metabolically active cells become available. Relatively few attempts been made with plant-associated species relate population size culturable cells, determined plating media, (9, 10, 74, 107). The occurrence a substantial proportion which not would serious implications disciplines pathology, ecology, phytoremediation.