Progress curve analysis of adenosine deaminase-catalyzed reactions
作者： Thomas Spector
DOI: 10.1016/0003-2697(84)90796-6
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摘要: Abstract The kinetic constants of the adenosine deaminase-catalyzed conversion to inosine were found be readily obtainable by analyzing progress curve a single reaction. A novel inhibitor, 9-(1-hydroxymethyl-3-methylbutyl)adenine, was studied test validity method with this enzyme. Estimates determined compared those conventional initial velocity analysis. Km and Vmax values for Ki value inhibitor estimated 26.1 μ m , 1.27 μmol/min/unit enzyme, 0.48 respectively, method, 29.3 0.52 shown act competitively substrate both methods experiments very simple perform calculated (with an interfaced microcomputer) within few minutes completion each assay.
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