The non-selective cation-permeable channel TRPC3 is a tetrahedron with a cap on the large cytoplasmic end.

摘要: TRPC3 plays important roles in neuronal differentiation and immune cell maturation by mediating the cationic current response to phospholipase C activation, Ca2+ depletion, diacylglycerol stimulation. Here, we purified channel using a glycosylated tetramer observed structure electron microscopy. Negatively stained specimens demonstrate homogeneous protein particles containing an internal cavity-like structure. These particle images were picked up automated pick-up programs, aligned, classified growing neural gas network method. Similarly oriented projections averaged decrease signal-to-noise ratio. The progress from top view side views, which are representative of their raw images. confirmed hypothesis four-domain structure, demonstrates large cytoplasmic domain with capped at bottom, is near predicted locus ion release. total image blunt-edged trapezoid 200 x 235 A. This dimension also supported Stokes radius (92 A) obtained gel filtration chromatography.