Synergistic Degradation of a Hyperuricemia-Causing Metabolite Using One-Pot Enzyme-Nanozyme Cascade Reactions.
作者： Secheon Jung , Inchan Kwon
DOI: 10.1038/SREP44330
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摘要: Multi-enzyme cascade reactions are frequently found in living organisms, particular when an intermediate should be eliminated. Recently, enzyme-mimic nanomaterials (nanozymes) received much attention for various applications, because they usually more stable and cost-effective than enzymes. However, enzyme-nanozyme reations have not been yet extensively exploited. Therefore, this study, we investigated one-pot using urate oxidase (UOX) catalase-mimic gold nanoparticle nanozyme (AuNP) with the ultimate goal of treatment hyperuricemia. UOX degrades hyperuricemia-causing uric acid, but also generates hydrogen peroxide raising several health concerns. We successfully demonstrated that UOX-AuNP systems degrade acid five times faster alone, by eliminating potentially cytotoxic peroxide, similar to enzyme-enzyme reactions.
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Yanyan Zhu, Bhavik J. Pandya, Hyon K. Choi, Prevalence of gout and hyperuricemia in the US general population: The National Health and Nutrition Examination Survey 2007–2008 Arthritis & Rheumatism. ,vol. 63, pp. 3136- 3141 ,(2011) , 10.1002/ART.30520
Hyon K. Choi, David B. Mount, Anthony M. Reginato, Pathogenesis of Gout Annals of Internal Medicine. ,vol. 143, pp. 499- 516 ,(2005) , 10.7326/0003-4819-143-7-200510040-00009
Hui Wei, Erkang Wang, Fe3O4 Magnetic Nanoparticles as Peroxidase Mimetics and Their Applications in H2O2 and Glucose Detection Analytical Chemistry. ,vol. 80, pp. 2250- 2254 ,(2008) , 10.1021/AC702203F
M. Ghadermarzi, A. A. Moosavi-Movahedi, Determination of the Kinetic Parameters for the “Suicide Substrate” Inactivation of Bovine Liver Catalase by Hydrogen Peroxide Journal of Enzyme Inhibition. ,vol. 10, pp. 167- 175 ,(1996) , 10.3109/14756369609030310
Xiaoli Liu, Yajun Xu, Zhaoqiang Wu, Hong Chen, Poly(N‐vinylpyrrolidone)‐Modified Surfaces for Biomedical Applications Macromolecular Bioscience. ,vol. 13, pp. 147- 154 ,(2013) , 10.1002/MABI.201200269
Min-Ah Woo, Moon Kim, Jae Jung, Ki Park, Tae Seo, Hyun Park, A novel colorimetric immunoassay utilizing the peroxidase mimicking activity of magnetic nanoparticles. International Journal of Molecular Sciences. ,vol. 14, pp. 9999- 10014 ,(2013) , 10.3390/IJMS14059999
Richard G. Zepp, Bruce C. Faust, Juerg Hoigne, Hydroxyl radical formation in aqueous reactions (pH 3-8) of iron(II) with hydrogen peroxide : the photo-fenton reaction Environmental Science & Technology. ,vol. 26, pp. 313- 319 ,(1992) , 10.1021/ES00026A011
Hui Wei, Erkang Wang, Nanomaterials with enzyme-like characteristics (nanozymes): next-generation artificial enzymes Chemical Society Reviews. ,vol. 42, pp. 6060- 6093 ,(2013) , 10.1039/C3CS35486E
Andrew N. Shipway, Michal Lahav, Rachel Gabai, Itamar Willner, Investigations into the Electrostatically Induced Aggregation of Au Nanoparticles Langmuir. ,vol. 16, pp. 8789- 8795 ,(2000) , 10.1021/LA000316K
Catherine J. Murphy, Anand M. Gole, Simona E. Hunyadi, John W. Stone, Patrick N. Sisco, Alaaldin Alkilany, Brian E. Kinard, Patrick Hankins, Chemical sensing and imaging with metallic nanorods. Chemical Communications. ,vol. 8, pp. 544- 557 ,(2008) , 10.1039/B711069C