Low-Intensity Laser Irradiation at 636 nm Induces Increased Viability and Proliferation in Isolated Lung Cancer Stem Cells
作者： Anine Crous , Heidi Abrahamse
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摘要: Abstract Objective: The purpose of this in vitro study was to evaluate the effects low-intensity laser irradiation (LILI) on isolated lung cancer stem cells (CSCs) after several time intervals, using a wavelength 636 nm and fluences between 5 20 J/cm2. Background data: LILI has been proven have biomodulatory effect various diseased conditions. A number studies conducted CSCs. Methods: Lung CSCs were from (A549), cell surface marker CD 133. Isolated divided into four groups: group 1 consisted control receiving no irradiation; groups 2, 3, 4 exposed at 5, 10, 20 J/cm2, respectively. performed diode with power output ±85 mW. Cellular responses evaluated 24, 48, or 72 h, included morphology, viability, proliferation. Results: morphology indicated an increase density caused by proliferation over ...
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