Characterizing gene expression responses to biomechanical strain in an in vitro model of osteoarthritis

摘要: Osteoarthritis (OA) is a common chronic degenerative joint disease affecting articular cartilage and underlying bone. Both genetic environmental factors appear to contribute the development of this disease. Specifically, pathological levels biomechanical stress on joints play notable role in initiation progression. Population-level gene expression studies cells experiencing may uncover gene-by-environment interactions relevant OA human health. To build foundation for such studies, we applied differentiation protocols develop an vitro system chondrogenic cell lines (iPSC-chondrocytes). We characterized regulatory responses three iPSC-chondrocyte cyclic tensile strain treatment. measured contribution biological technical variation and, even small sample, found several genes that exhibit inter-individual differences response mechanical strain, including previously implicated OA. Expanding include iPSC-chondrocytes from larger number individuals will allow us characterize better understand related