Characterization of growth hormone receptor messenger ribonucleic acid variants in human adipocytes.

摘要: Context: Human GH exerts profound effects on adiposity through its specific receptor, hGHR. Eight hGHR mRNAs are produced by the gene due to splicing from alternate 5′-untranslated region first exons into a common acceptor site upstream of start codon in exon 2. Four transcripts (V2, V3, V5, V9) ubiquitously expressed, whereas other four (V1, V4, V7, V8) expressed only normal postnatal liver, suggesting that different promoter usage is mechanism for developmental- and tissue-specific regulation gene. Objective: Because it unknown whether this occurs adipocytes, we screened human adipocyte cDNA using 5′-rapid amplification ends. Results: Eighty-nine percent clones were V2-like, 3% V3-like, 8% five new mRNA variants (VA-VE). All sequences mapped within 5′flanking region. RT-PCR assays showed expression multiple fetal adult tissues, and, thus, they not specific. We ...